🔵 Actin

easymode segment actin

The actin model was trained to output a cylindrical tube with a diameter of 60 Å along actin filaments. It was one of the most challenging models to train thus far and remains somewhat experimental.

In combination with the --filament flag in easymode pick, the model enables tracing actin filaments and picking of particles at regular intervals along individual filaments, with an accurate prior on the particle orientation and class labels linking particles to the parent filaments (_aisFilamentID). Use the --per-filament flag to write .star files for individual actin filaments.

Example output

Example of easymode segment actin output overlaid on a tomogram from training dataset 001_HELA. We have yet to validate the model on unseen data (by subtomogram averaging)